
C3 - Prof. Dr. Andreas Herrmann/ PD Dr. Michael Veit
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C3: Specific recruitment of viral components and assembly of enveloped viruses
Employing biophysical, biochemical and reverse genetic approaches, we will analyse two processes essential for assembly and budding of Influenza virus: Recruitment of the envelope protein M2 and the coat protein M1 to the viral budding site and the generation of membrane curvature induced by oligomerization of M1 and membrane insertion of an amphiphilic helix of M2. Viruses must be assembled in such a way that this assembly can be reversed later on in subsequent infections in a coordinated, serial manner. The results will provide a molecular understanding how the functions each protein has for both processes are regulated.
Selected Publications
- Authors:Wang M, Veit M
Journal:Cell Microbiol Year:2016; Volume:18Issue:(5):Pages:692-704. doi: 10.1111/cmi.12541. Epub 2015 Nov 23.
Title:Hemagglutinin-esterase-fusion (HEF) protein of influenza C virus - Authors:Scolari S, Imkeller K, Jolmes F, Veit M, Herrmann A, Schwarzer R
Journal:Cell Microbiol Year:2016; Volume:18Issue:(1):Pages:125-36. doi: 10.1111/cmi.12491. Epub 2015 Aug 31.
Title:Modulation of cell surface transport and lipid raft localization by the cytoplasmic tail of the influenza virus hemagglutinin - Authors:Veit M, Siche S
Journal:Vaccine Year:2016; Volume:33Issue:(49):Pages:7002-7. doi: 10.1016/j.vaccine.2015.08.095. Epub 2015 Sep 19.
Title:S-acylation of influenza virus proteins: Are enzymes for fatty acid attachment promising drug targets? - Authors:Wang M, Ludwig K, Böttcher C, Veit M
Journal:Cell Year:2016; Volume:18Issue:(5):Pages:92-704. doi: 10.1111/cmi.12541. Epub 2015 Nov 23.
Title:The role of stearate attachment to the hemagglutinin-esterase-fusion glycoprotein HEF of influenza C virus - Authors:Höfer CT, Jolmes F, Haralampiev I, Veit M, Herrmann A
Journal:Cell Microbiol Year:2016; Volume:4:Pages: doi: 10.1111/cmi.12679. Epub 2016 Oct 26.
Title:Influenza A virus nucleoprotein targets subnuclear structures - Authors:Siche S, Brett K, Möller L, Kordyukova LV, Mintaev RR, Alexeevski AV, Veit M
Journal:Viruses Year:2015; Volume:7Issue:(12):Pages:6458-75. doi: 10.3390/v7122950.
Title:Two Cytoplasmic Acylation Sites and an Adjacent Hydrophobic Residue, but No Other Conserved Amino Acids in the Cytoplasmic Tail of HA from Influenza A Virus Are Crucial for Virus Replication - Authors:Hilsch, M., Goldenbogen, B., Sieben, C., Höfer, C.T., Rabe, J.P., Klipp, E., Herrmann, A., Chiantia, S
Journal:Biophys Year:2014; Volume:107Issue:(4):Pages:912-23. doi: 10.1016/j.bpj.2014.06.042.
Title:Influenza A matrix protein M1 multimerizes upon binding to lipid membranes - Authors:Thaa, B, Siche, S, Herrmann, A, and Veit, M
Journal:FEBS Lett Year:2014; Volume:588Issue:(6):Pages:1031-36.
Title:Acylation and cholesterol binding are not required for targeting of influenza A virus M2 pro-tein to the hemagglutinin-defined budozone - Authors:Mair CM, Meyer T, Schneider K, Huang Q, Veit M, Herrmann A
Journal:J Virol Year:2014; Volume:88Issue:(22):Pages:13189-200. doi: 10.1128/JVI.01704-14. Epub 2014 Sep 3.
Title:A histidine residue of the influenza virus hemagglutinin controls the pH dependence of the conformational change mediating membrane fusion - Authors:de Vries M, Herrmann A, Veit M
Journal:Biochem J Year:2014; Volume:465Issue:(2):Pages:305-14. doi: 10.1042/BJ20141114.
Title:A cholesterol consensus motif is required for efficient intracellular transport and raft association of a group 2 HA from influenza virus - Authors:Brett K, Kordyukova LV, Serebryakova MV, Mintaev RR, Alexeevski AV, Veit M
Journal:J Biol Chem Year:2014; Volume:289Issue:(50):Pages:34978-89. doi: 10.1074/jbc.M114.586180. Epub 2014 Oct 27.
Title:Site-specific S-acylation of influenza virus hemagglutinin: the location of the acylation site relative to the membrane border is the decisive factor for attachment of stearate - Authors:Schwarzer, R, Gramatica, A, Levental, I, Scolari, S, Buschmann, V, Veit, M, and Herrmann, A.
Journal:Cell Microbiol Year:2014; :Pages:doi:10.1111/cmi.12314.
Title:The cholesterol-binding motif of the HIV-1 glycoprotein gp41 regulates lateral sorting and ol-igomerization - Authors:Schmitt, FJ, Thaa, B, Junghans, C, Vitali, M, Veit, M, and Friedrich, T
Journal:Biochim Biophys Acta pii Year:2014; :Pages:S0005-2728(14)00110-8.
Title:eGFP-pHsens as a highly sensitive fluorophore for cellular pH determination by fluores-cence lifetime imaging microscopy (FLIM) - Authors:Kummer, S, Flöttmann, M, Schwanhäusser, B, Sieben, C, Veit, M, Selbach, M, Klipp, E*, and Herrmann, A
Journal:PLoS One Year:2014; Volume:9Issue:(4):Pages:e94257.
Title:Alteration of protein levels during Influenza virus H1N1 infection in host cells: A proteomic survey of host and virus reveals differential dynamics - Authors:Li, S, Sieben, C, Ludwig, K, Herrmann, A*, Eghiaian, F*, Schaap, IAT
Journal:Biophys J Year:2014; Volume:106Issue:(7):Pages:1447-56.
Title:pH-controlled two-step uncoating of influenza virus - Authors:Kordyukova LV, Serebryakova, MV and Veit M
Journal:Biochemical Society Transactions Year:2013; Volume:41:Pages:50-55.
Title:Palmitoylation of influenza virus proteins - Authors:Veit, M
Journal:Biology of the Cell Year:2012; Volume:104:Pages:493-515.
Title:Palmitoylation of virus proteins - Authors:Veit M, Engel S, Thaa B, Scolari S and Herrmann A
Journal:Cellular Microbiology Year:2013; Volume:15Issue:(2):Pages:179-189.
Title:Lipid domain association of influenza virus proteins detected by dynamic fluorescence microscopy techniques - Authors:Kabatak, A., Veit, M.
Journal:Viruses Year:2012; Volume:4Issue:(3):Pages:414-23.
Title:Folding and Oligomerization of the gp2b/gp3/gp4 Spike Proteins of Equine Arteritis Virus in Vitro - Authors:Engel, S., de Vries, M., Herrmann, A., Veit, M.
Journal:FEBS Lett Year:2012; Volume:586Issue:(3):Pages:277-82.
Title:Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi - Authors:Veit, M., Thaa, B.
Journal:Adv Virol Year:2011; :Pages:doi: 10.1155/2011/370606.
Title:Association of influenza virus protein with membrane rafts. Review Article. Advances in Virology - Authors:Thaa, B., Levental, I., Herrmann, A., Veith, M. 437,
Journal:Biochem J Year:2011; Volume:437:Pages:389-397.
Title:Intrinsic membrane association of the cytoplasmic tail of influenza virus M2 protein and lateral membrane sorting regulated by cholesterol binding and palmitoylation - Authors:Kordyukova, L.V., Serebryakova, M.V., Polyansky, A.A., Kropotkina, E.A., Alexeevski, A.V., Veit, M., Efremov, R.G., Filippova, I.Y., Baratova, L.A.
Journal:Biochim Biophys Acta Year:2011; Volume:1808Issue:(7):Pages:1843-54.
Title:Linker and/or transmembrane regions of influenza A/Group-1, A/Group-2, and type B virus hemagglutinins are packed differently within trimers - Authors:Thaa, B., Levental, I., Herrmann, A., Veith, M. 437,
Journal:Biochem J Year:2011; Volume:437:Pages:389-397.
Title:Intrinsic membrane association of the cytoplasmic tail of influenza virus M2 protein and lateral membrane sorting regulated by cholesterol binding and palmitoylation - Authors:Serebryakova, M.V., Kordyukova, T. A. Semashko, T.A., Ksenofontov, A.L., Rudneva, I.A., Kropotkina, E.A., Filippova, I.Y., Veit, M, Baratova, L.A.
Journal:Virus Research Year:2011; Volume:160Issue:(1-2):Pages:294-304.
Title:Influenza virus hemagglutinin spike neck architectures and interaction with model enzymes evaluated by MALDI-TOF mass spectrometry and bioinformatic tools - Authors:Thaa B, Tielesch C, Möller L, Schmitt, AO, Wolff, T, Bannert N, Herrmann A, Veit M
Journal:J Gen Virol Year:2011; Volume:93Issue:(2):Pages:282-92.
Title:Growth of influenza A virus is not impeded by simultaneous removal of the cholesterol binding and acylation sites in the M2 protein - Authors:Barberis M, Beck C, Amoussouvi A, Schreiber G, Diener C, Herrmann A, Klipp E
Journal: Mol Biosyst Year:2011; Volume:7Issue:(10):Pages:2804-12.
Title:A low number of SIC1 mRNA molecules ensures a low noise level in cell cycle progression of budding yeast - Authors:Schreiber G, Barberis M, Scolari S, Klaus C, Herrmann A, Klipp E. (2011) :
Journal:FASEB J Year:2011; Volume:26Issue:(2):Pages:546-54.
Title:Unraveling interactions of cell cycle-regulating proteins Sic1 and B-type cyclins in living yeast cells: a FLIM-FRET approach