
A4 - Prof. Dr. Markus Wahl
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A4: The sub-module for spliceosome catalytic activation and disassembly
Using integrative structural biochemistry, project A4 will investigate the architecture of a large spliceosomal submodule, which consists of the Brr2 RNA helicase, the Snu114 G-protein and the Prp8 regulatory scaffold and which is required for RNP remodeling events that elicit spliceosome catalytic activation, as well as of its substrate complex. We will then analyze how molecular, submodular and modular functions of the spliceosome are affected by structure-guided site-directed mutagenesis and will also investigate how the submodule is regulated by reversible ubiquitination.
Selected Publications
- Authors:Theuser M, Höbartner C, Wahl MC, Santos KF
Journal:Proc Natl Acad Sci U S A. Year:2016; Volume:113Issue:(28):Pages:7798-803. doi: 10.1073/pnas.1524616113. Epub 2016 Jun 27.
Title:Substrate-assisted mechanism of RNP disruption by the spliceosomal Brr2 RNA helicase - Authors:Absmeier E, Santos KF, Wahl MC
Journal:Cell Cycle Year:2016; Volume:15Issue:(24):Pages:3362-3377. doi: 10.1080/15384101.2016.1249549. Epub 2016 Oct 28.
Title:Function and regulation of the Brr2 RNA helicase during splicing - Authors:Wahl MC, Fischer U
Journal:Genes Dev Year:2016; Volume:30Issue:(21):Pages:2341-2344.
Title:The right pick: Structural basis of snRNA selection by Gemin5 - Authors:Absmeier E, Becke C, Wollenhaupt J, Santos KF, Wahl MC
Journal:Cell Cycle Year:2016; Volume:16Issue:(1):Pages:100-112. doi: 10.1080/15384101.2016.1255384. Epub 2016 Nov 23.
Title:Interplay of cis- and trans-regulatory mechanisms in the spliceosomal RNA helicase Brr2 - Authors:Absmeier E, Rosenberger l, Apelt L, Becke C, Santos KF, Stelzl U, Wahl MC
Journal:Acta Crystallogr D Year:2015; Volume:71:Pages:762-771.
Title:A non-canonical PWI domain in the N-terminal helicase-associated region of the spliceosomal Brr2 protein - Authors:Liu S, Mozaffari-Jovin S, Wollenhaupt J, Santos KF, Theuser M, Dunin-Horkawicz S, Fabrizio P, Bujnicki JM, Lührmann R, Wahl MC
Journal:eLife Year:2015; Volume:4Issue:(e07320):Pages:doi: 10.7554/eLife.07320.
Title:A composite, double-stranded/single-stranded RNA-binding region in the Prp3 protein important for U4/U6•U5 tri-snRNP stability and splicing - Authors:Absmeier E, Wollenhaupt J, Mozaffari-Jovin S, Becke C, Lee CT, Marco Preussner M, Heyd F, Urlaub H, Lührmann R, Santos KF, Wahl MC
Journal:Genes Dev Year:2015; Volume:29Issue:(24):Pages:2576-87. doi: 10.1101/gad.271528.115.
Title:The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation - Authors:Mozaffari-Jovin, S, Wandersleben, T, Santos, KF, Will, CL, Lührmann, R and Wahl, MC
Journal:RNA Biol Year:2014; Volume:11Issue:(4):Pages:298-312.
Title:Novel regulatory principles of the spliceosomal Brr2 RNA helicase and links to retinal dis-ease in humans - Authors:Mozaffari-Jovin S, Wandersleben T, Santos KF, Will CL, Lührmann R, Wahl MC
Journal:Science Year:2013; Volume:341Issue:(6141):Pages:80-4.
Title:Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8 - Authors:Weber G, Cristão VF, Santos KF, Jovin SM, Heroven AC, Holton N, Lührmann R, Beggs JD, Wahl MC
Journal:Genes Dev Year:2013; Volume:27Issue:(5):Pages:525-40.
Title:Structural basis for dual roles of Aar2p in U5 snRNP assembly - Authors:Pühringer, S, Hellmig, M, Liu, S, Weiss, MS, Wahl, MC and Müller, U
Journal:Acta Crystallogr F 68 Year:2012; Volume:68:Pages:981-4.
Title:Structure determination by multiple-wavelength anomalous dispersion (MAD) at the Pr LIII edge - Authors:Santos KF, Jovin SM, Weber G, Pena V, Lührmann R, Wahl MC
Journal:Proc Natl Acad Sci U S A Year:2012; Volume:109Issue:(43):Pages:17418-23.
Title:Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome - Authors:Mozaffari-Jovin S, Santos KF, Hsiao HH, Will CL, Urlaub H, Wahl MC, Lührmann R
Journal:Genes Dev Year:2012; Volume:26Issue:(21):Pages:2422-34.
Title:The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA - Authors:Liu, S., Ghalei, H., Lührmann, R., Wahl, M.C.
Journal:RNA Year:2011; Volume:17:Pages:1655-1663.
Title:Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31 - Authors:Weber, G., Cristão, V.F., de L Alves, F., Santos, K.F., Holton, N., Rappsilber, J., Beggs, J.D., Wahl, M.C.
Journal:Genes Dev Year:2011; Volume:25:Pages:1601-1612.
Title:Mechanism for Aar2p function as a U5 snRNP assembly factor