Publications Subproject Z1:
Molecular electron microscopy and electron tomography

Connell SR, Topf T, Qin Y, Wilson DN, Mielke T, Fucini P, Nierhaus KH and Spahn CMT. A novel tRNA-intermediate revealed on the ribosome during EF4-mediated back-translocation. Nature Struct Mol Biol. (2008) in revision

Harms JM, Wilson DN, Schluenzen F, Connell SR, Stachelhaus T, Zaborowska Z, Spahn CMT, Fucini P. Translational regulation via L11: Molecular switches on the ribosome turned on and off by the antibiotics thiostrepton and micrococcin. Mol Cell. (2008) in press (IF 16,8)

Chen J, Sachse C, Xu C, Mielke T, Spahn CMT and Grigorieff N. A Dose-Rate Effect in Single-Particle Electron Microscopy. J. Struc. Biol. 161 (2008) 92-100 [Link]

Connnell SR, Takemoto C, Wilson DN, Wang H, Murayama K, Terada T, Shirouzu M, Rost M, Schüler M, Giesebrecht J, Dabrowski M, Mielke T, Fucini PM, Yokoyama S and Spahn CMT. Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors. Molecular Cell 25 (2007) 751-764. [Link]

Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R, Cramer P. Functional architecture of RNA polymerase I. Cell 131 (2007) 1260-1272. [Link]

Andersen CBF, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Petersen JS, Spahn CMT, Goss Kinzy T, Andersen GR and Beckmann R. Structure of eEF3 and the mechanism of transfer RNA release from the E-site. Nature 443 (2006) 663-668c. [Link]

Schüler M, Connell SR, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, Mielke T, Penczek PA, Westhof E, Spahn CMT. Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nature Structural & Molecular Biology 13 (2006) 1092-1096. [Link]

Penczek PA, Yang C, Frank J, Spahn CMT. Estimation of variance in single particle reconstruction using the bootstrap technique. J Struct Biol 154 (2006) 168-183 [Link]

Penczek PA, Frank J, Spahn CMT. A method of focused classification, based on the bootstrap 3-D variance analysis, and its application to EF-G-dependent translocation. J Struct Biol 154 (2006) 184-194 [Link]

Halic, M., Gartmann, M., Schlenker, O., Mielke, T., Pool, M.R., Sinning, I. and Beckmann, R. Signal Recognition Particle Receptor Exposes the Ribosomal Translocon Binding Site. Science 312 (2006) 745-747 [Link]

Halic M, Becker T, Frank J, Spahn CMT, Beckmann R. Localization and dynamic behavior of ribosomal protein L30e. Nat Struct Mol Biol 12 (2005) 467-468 [Link]

Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Silasagaram A, Spahn CMT, Grassucci RA, Kaji A, Frank J. Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications. Proc Natl Acad Sci USA 101 (2004) 8900-8905 [Link]

Ruprecht J, Mielke T, Vogel R, Villa C, Schertler GF. Electron crystallography reveals the structure of metarhodopsin I. EMBO J. 23 (2004) 3609–3620 [Link]

Spahn CMT, Gomez-Lorenzo MG, Grassucci RA, Jørgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JPG, Frank J. Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation. EMBO J 23 (2004) 1008-1019 [Link]

Halic M, Becker T, Pool M, Spahn CMT, Grassucci RA, Frank J, Beckmann R. Structure of the Signal Recognition Particle interacting with the Elongation Arrested Ribosome. Nature 427 (2004) 808-814 [Link]

Dr. Thorsten Mielke
Prof. Dr. Christian M. T. Spahn

Gomez-Lorenzo MG, Spahn CMT, Agrawal RK, Grassucci RA, Penczek P, Chakraburtty K, Ballesta JPG, Lavandera JL, Garcia-Bustos JF, Frank J. Three-dimensional Cryo-EM Localization of EF2 in the Saccharomyces cerevisiae 80S Ribosome at 17.5 Å Resolution. EMBO J 19 (2000) 2710-2718 [Link]

Spahn CMT, Jan E, Mulder A, Grassucci RA, Sarnow P, Frank J. Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor. Cell 118 (2004) 465-475 [Link]

Sengupta J, Nilsson J, Gursky R, Spahn CMT, Nissen P, Frank J. Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM. Nat Struct Mol Biol 11 (2004) 957-962 [Link]

Vogel R, Ruprecht J, Villa C, Mielke T, Schertler GF, Siebert F. Rhodopsin Photoproducts in 2D Crystals. J. Mol. Biol. 338 (2004) 597–609 [Link]

Mielke T, Villa C, Edwards PC, Schertler GF, Heyn MP. X-ray diffraction of heavy-atom labelled two-dimensional crystals of rhodopsin identifies the position of cysteine 140 in helix 3 and cysteine 316 in helix 8. J. Mol. Biol. 316 (2002) 693-709 [Link]

Gao H, Spahn CMT, Grassucci RA, Frank J. An assay for local quality in cryo-electron micrographs of single particles. Ultramicroscopy 93 (2002)169-178 [Link]

Beckmann R, Spahn CMT, Narayanan E, Helmers J, Penczek P, Sali A, Frank J, Blobel G. Analysis of the protein-conducting channel associated with the translating 80S ribosome. Cell 107 (2001) 361-372 [Link]

Spahn CMT, Blaha G, Agrawal RK, Penczek P, Grassucci RA, Trieber CA, Connell SR, Taylor DE, Nierhaus KH, Frank J. Localization of the ribosomal protection protein Tet(O) on the ribosome and the mechanism of tetracycline resistance. Mol Cell 7 (2001) 1037-1045 [Link]

Spahn CMT, Beckmann R, Narayanan E, Penczek PA, Sali A, Blobel G, Frank J. tRNA-ribosome and subunit–subunit interactions within the eukaryotic 80S ribosome from Sacharomyces cerevisiae. Cell 107 (2001) 373-386 [Link]

Spahn CMT, Kieft JS, Grassucci RA, Penczek P, Zhou K, Doudna A, Frank J. Hepatitis C virus IRES RNA induced changes in the conformation of the 40S subunit. Science 291 (2001) 1959-1962 [Link]

Agrawal RK, Spahn CMT, Penczek P, Grassucci RA, Nierhaus KH, Frank J. Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle. J Cell Biol 150 (2000) 447-459 [Link]

Gabashvili IS, Agrawal RK, Spahn CMT, Grassucci RA, Svergun DI, Frank J, Penczek P. Solution structure of the E. coli 70S ribosome at 11.5 A resolution. Cell 100 (2000) 537-549 [Link]

Spahn CMT, Penczek PA, Leith A, Frank J. A method for differentiating proteins from nucleic acids in intermediate-resolution density maps: cryo-electron microscopy defines the quaternary structure of the Escherichia coli 70S ribosome. Structure Fold Design 8 (2000) 937-948 [Link]

Spahn CMT, Grassucci RA, Penczek P, Frank J. Direct three-dimensional localization and positive identification of RNA helices within the ribosome by means of genetic tagging and cryo-electron microscopy. Structure Fold Des 7 (1999) 1567-1573 [Link]